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KMID : 0350519930460041655
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Journal of Catholic Medical College
1993 Volume.46 No. 4 p.1655 ~ p.1665
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Human Papillomavirus Detection Using Polymerase Chain Reation in Cervical Cancer Tissue and Paraaortic Lymph Nodes
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Abstract
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Tissues of forty-five cervical cancers and paraaortic lymph nodes from the same patients were evaluated by polymerase chain reacition with L1 consensus primers and hybridization with type-specific oligomer probes of HPV-6, -11, -16, -18, -31,
-33,
-35,
and -45 for the detection and classification of subtypes of human papillomavirus(HPV). The clinicopathologic findings of surgical specimens from radical hysterectomy were compared with the results of HPV detection to use as a possible prognostic
marker
for the early detection of paraaortic nodes involvement. Metastasis to paraaortic nodes in cervical cancer, suggesting extrapelvic involvement of tumor, is clinically important to predict prognosis of the patients.
The overall detection rate of HPV DNA in cervical cancer tissue was 93 % Among HPV sub types, HPV -16 was the most frequent in cervical cancers(76 %, P<0.01). Five tumors were positive for HPV -18, two for HPV -33, one for HPV -45 and onel for
unidentified type of HPV, respectively. HPV -18 was detected more frequently in adenocacinoma than in squamous carcinoma(P<0.05). No HPV DNA was detected in 3 cases of cervical cancer. HPV DNA were also detected in fourteen cases(31 %), 13 cases
of
HPV
-16 and 1 case of HPV -18, out of forty-five histologically negative paraaortic lymph nodes. HPV DNA in histologically negative paraaortic lymph nodes suggested biologic metastasis and might play an important role in decision of postoperative
adjuvant
treatment.
These results provided further evidence implicating HPV in the etiolgy of cervical cancer and its metastasis and recommending PCR as a sensitive method for the detection of HPV DNA positive cells. The prognostic significance of HPV DNA in
histologically
negative paraaortic lymph nodes remains to be established after several years of follow-up.
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KEYWORD
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